Tag: quantification

A practical pipeline for turning your own bulk RNA-seq FASTQs into quantified gene and transcript expression, with the tool choices, flags, QC thresholds, and failure modes that matter for a single-person longitudinal dataset.

A working guide to turning personal RNA sequencing FASTQs into gene- and transcript-level expression estimates: reference choice, selective alignment with Salmon, spliced alignment with STAR, QC thresholds, and the checks that catch a swapped or degraded sample.