Tag: pca

2 posts

How to Plot Your Own RNA-Seq Data: PCA, Volcano, and Trajectory Graphs

September 19, 2026

A working guide to turning RNA-seq FASTQs into the handful of plots that matter: sample PCA, distance heatmaps, MA and volcano plots, and longitudinal gene trajectories, with the commands, parameters, and failure modes.

rna-seq pca visualization deseq2 python

How to Make and Read a PCA Plot for RNA-seq Data

September 18, 2026

A practical guide to computing principal components from RNA-seq counts in R: which transform to use, how many genes to feed in, how to figure out what each component means, and when PCA is the wrong tool.

rna-seq pca deseq2 quality-control r