Tag: grch38

A working guide to lifting positions, VCFs, intervals, and probe manifests between GRCh37, GRCh38, and T2T-CHM13, including which tool to use for each file type and how to tell when the lift silently went wrong.

A practical guide to picking a human genome build and annotation for RNA-seq, building STAR and salmon indexes correctly, and checking that the reference you built is the one your quantification assumes.